An optic-fiber graphene field effect transistor biosensor for the detection of single-stranded DNA. Issue 15 (29th March 2021)

• Record Type: Journal Article
• Authors: Zhang, Yanan; Ding, Yue; Li, Can; Xu, Huaqiang; Liu, Chunxiang; Wang, Jingjing; Ma, Yong; Ren, Junfeng; Zhao, Yuefeng; Yue, Weiwei
• Is Part Of: Analytical methods. Volume 13:Issue 15(2021)
• Page Start: 1839
• Page End: 1846
• Publication Date: 2021-03-29
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d1ay00101a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Herein, a graphene field effect transistor (GFET) was constructed on an optical fiber end face to develop an integrated optical/electrical double read-out biosensor, which was used to detect target single-stranded DNA (tDNA). Abstract : Herein, a graphene field effect transistor (GFET) was constructed on an optic fiber end face to develop an integrated optical/electrical double read-out biosensor, which was used to detect target single-stranded DNA (tDNA). Two isolated Au electrodes were, respectively, prepared as the drain and source at the ends of an optic fiber and coated with a graphene film to construct a field effect transistor (FET). Probe aptamers modified with fluorophore 6′-carboxy-fluorescein (6′-FAM) were immobilized on the graphene for specific capture of tDNA. Graphene oxide (GO) was introduced to quench 6′-FAM and construct a fluorescence biosensor. Thus, a dual GFET and fluorescence biosensor was integrated on the end-face of an optic fiber. Following synchronous detection by fluorescence and FET methods, results showed satisfactory sensitivity for DNA detection. Compared with conventional biosensors using a single sensing technology, these dual sensing integrated biosensors significantly improved the reliability and accuracy of DNA detection. Furthermore, this proposed technique provides both a new biosensor for single-stranded DNA detection and a strategy for designing multi-sensing integrated biosensors.