Graphene based electrochemical immunosensor for the ultra-sensitive label free detection of Alzheimer's beta amyloid peptides Aβ(1–42). Issue 8 (4th March 2021)

• Record Type: Journal Article
• Authors: Abbasi, Hina. Y.; Tehrani, Zari; Devadoss, Anitha; Ali, Muhammad Munem; Moradi-Bachiller, Soraya; Albani, Diego; Guy, Owen. J.
• Is Part Of: Nanoscale advances. Volume 3:Issue 8(2021)
• Page Start: 2295
• Page End: 2304
• Publication Date: 2021-03-04
• Subjects: 620.5
• DOI: 10.1039/d0na00801j
• Languages: English
• ISSNs: 2516-0230

Abstract

Abstract : Development of ultra-thin polymer layer modified screen printed graphene electrodes with high conductivity for higher-sensitivity detection of Aβ(1–42) peptides with the lowest LOD of 1.4 pg mL −1 is presented. Abstract : An immunosensor capable of high sensitivity detection of beta-amyloid peptides, shown to be a reliable biomarker for Alzheimer's disease, has been developed using screen printed graphene electrodes (SPGEs) modified with ultra-thin layers of polymerised 1, 5-diaminonaphthalene (pDAN). Electropolymerization of 1, 5-diaminonaphthalene (DAN) was performed to coat the graphene screen printed electrodes in a continuous polymer layer with controlled thickness. The surface characteristics of pristine graphene and polymer modified graphene electrodes were examined using Raman and X-ray photoelectron spectroscopy. The effects of polymer thickness on the electron transfer rates were investigated. An immunosensor for selective detection of beta amyloid peptides Aβ(1–42) was developed via biofunctionalization of the pDAN modified SPGE with the anti-beta amyloid antibody used as the peptide bioreceptor. The immunosensor has been used for specific detection of Aβ(1–42) with a linear range of 1 pg mL −1 to 1000 pg mL −1 and showed 1.4 pg mL −1 and 4.25 pg mL −1 detection and quantification limit, respectively. The biosensor was further validated for the analysis of spiked human plasma. The immunosensor enables rapid, accurate, precise, reproducible and highly sensitive detection of Aβ(1–42) using a low-cost SPGE platform, which opens the possibilities for diagnostic ex vivo applications and research-based real time studies.