Rapid determination of hexapeptides by hydrophilic interaction LC–MS/MS for in vitro skin-penetration studies. (June 2013)

• Record Type: Journal Article
• Authors: Zhou, Wanlong; Wang, Perry G; Ogunsola, Oluwatosin A; Kraeling, Margaret EK
• Is Part Of: Bioanalysis. Volume 5:Number 11(2013)
• Page Start: 1353
• Page End: 1362
• Publication Date: 2013-06
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.13.60
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: A sensitive analytical method is needed for assessing penetration of topically applied peptides for in vitro skin-penetration studies.Results: A rapid hydrophilic interaction LC (HILIC)–MS/MS method for analyzing the polar peptides Ac-EEMQRR-amide and H2 N-EEMQRR-amide in various skin layers and matrices has been developed and evaluated. The matrices included emulsion, receptor fluids, cotton-tipped applicators, stratum corneum tape strips, epidermis and dermis of the skin. Stable isotopically labeled analogues were used as internal standards to correct for recovery and matrix effects. A HILIC-SPE procedure was optimized to minimize significant ion suppression in the more complex matrices.Conclusion: This HILIC–MS/MS method is applicable to the determination of Ac-EEMQRR-amide and H2 N-EEMQRR-amide in complex skin samples and other matrices generated during in vitro skin-penetration studies.