Hepatotoxicity and nephrotoxicity in rats induced by carbon tetrachloride and the protective effects of Teucrium polium and vitamin C. (13th June 2019)

• Record Type: Journal Article
• Authors: Rahmouni, Fatma; Badraoui, Riadh; Amri, Nahed; Elleuch, Aïda; El-Feki, Abdelfattah; Rebai, Tarek; Saoudi, Mongi
• Is Part Of: Toxicology mechanisms and methods. Volume 29:Number 5(2019)
• Page Start: 313
• Page End: 321
• Publication Date: 2019-06-13
• Subjects: Carbon tetrachloride -- oxidative stress -- vitamin C -- Teucrium polium; Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.1080/15376516.2018.1519864
• Languages: English
• ISSNs: 1537-6516

Abstract

Abstract: This work investigated the protective effects of Teucrium polium ( T. polium ) and vitamin C (Vit C) against carbon tetrachloride (CCl4 ) induced hepatotoxicity and nephrotoxicity in rats. T. polium reduced the Fer reduced antioxidant power (FRAP) (IC50 = 0.89 mg/ml) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) (IC50 = 0.049 µg/ml) than Vit C, FRAP (IC50 = 0.71 mg/ml) and DPPH (IC50 = 0.029 µg/ml). Male albino Wistar rats were divided into six groups: Group I was used as controls, Group II received CCl4 in olive oil (0.5 ml/kg) by gavage, Group III received CCl4 in olive oil (0.5 ml/kg) by gavage after 3 d of receiving T. polium (5 g/l), orally, Group IV received T. polium (5 g/l) alone, by gavage, for 7 d, Group V received CCl4 in olive oil (0.5 ml/kg) by gavage after 3 d of receiving Vit C (250 mg/kg) by gavage and Group VI received Vit C (250 mg/kg) alone by gavage. CCl4 showed an increase of serum hepatic and renal markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine. Moreover, we noted an increase of lipid peroxidations and a decrease in antioxidants enzymes: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) of CCl4 rats compared to controls. The pretreatment with (200 mg/kg) of T. polium and with Vit C (250 mg/kg) by gavage, for 7 d, displayed their ability to protect against oxidative damage and biochemical changes induced by CCl4 . Our results were in accordance with histopathological observations. Graphical Abstract: