Biphasic and triphasic dose responses in zebrafish embryos to low-dose 150 kV X-rays with different levels of hardness. (3rd August 2016)

• Record Type: Journal Article
• Authors: Kong, Eva Yi; Cheng, Shuk Han; Yu, Kwan Ngok
• Is Part Of: Journal of radiation research. Volume 57:Number 4(2016:Jul.)
• Page Start: 363
• Page End: 369
• Publication Date: 2016-08-03
• Subjects: Radiology, Medical -- Periodicals; Radiobiology -- Periodicals; Radiation -- Periodicals; 616.0757
• DOI: 10.1093/jrr/rrw026
• Languages: English
• ISSNs: 0449-3060

Abstract

Abstract: The in vivo low-dose responses of zebrafish ( Danio rerio ) embryos to 150 kV X-rays with different levels of hardness were examined through the number of apoptotic events revealed at 24 h post fertilization by vital dye acridine orange staining. Our results suggested that a triphasic dose response was likely a common phenomenon in living organisms irradiated by X-rays, which comprised an ultra-low-dose inhibition, low-dose stimulation and high-dose inhibition. Our results also suggested that the hormetic zone (or the stimulation zone) was shifted towards lower doses with application of filters. The non-detection of a triphasic dose response in previous experiments could likely be attributed to the use of hard X-rays, which shifted the hormetic zone into an unmonitored ultra-low-dose region. In such cases where the subhormetic zone was missed, a biphasic dose response would be reported instead.