L-Carnitine activates calcium signaling in human osteoblasts. (August 2018)

• Record Type: Journal Article
• Authors: Ferraretto, Anita; Bottani, Michela; Villa, Isabella; Giusto, Lucia; Signo, Michela; Senesi, Pamela; Montesano, Anna; Vacante, Fernanda; Luzi, Livio; Rubinacci, Alessandro; Terruzzi, Ileana
• Is Part Of: Journal of functional foods. Volume 47(2018)
• Page Start: 270
• Page End: 278
• Publication Date: 2018-08
• Subjects: [Ca2+]i intracellular calcium concentration -- [Ca2+]o extracellular calcium concentration -- CaSR Calcium Sensing Receptor -- CCCP carbonyl cyanide 3-chlorophenylhydrazone -- DiBAC4-(3) bis-(1, 3-dibutylbarbituric acid) trimethine oxonol -- ER endoplasmic reticulum -- FBS fetal bovine serum -- hOBs human osteoblast-like cells -- IMDM Iscove's modified medium -- L-C L-Carnitine -- L-VDCC L-type voltage dependent calcium channels -- PLC phospholipase C -- Tg thapsigargin; L-Carnitine -- Human primary osteoblast-like cells -- L-VDCC -- PLC sensitive stores -- Mitochondrial calcium stores -- Calcium sensing receptor; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.05.068
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: L-C induces calcium signaling in human osteoblasts. L-C induces calcium entry from plasma membrane depolarizing channels. L-C induces a calcium release by cytoplasmic stores. The L-C induced calcium signaling is mediated by CaSR. Abstract: L-Carnitine (L-C) is a conditionally essential nutrient required for β-oxidation and cell energy production whose unbalanced metabolism is associated with a variety of diseases. L-C increased cell proliferation, differentiation and mineralization of human osteoblast in culture. The present study evaluated the effect of L-C on intracellular calcium signaling, an important modulator of cell proliferation and differentiation in human osteoblasts by means of Fura-2 fluorescence coupled to video imaging technique. 5 mM L-C promoted a calcium influx from the extracellular milieu by depolarizing L-type calcium channels. Moreover, L-C depleted the calcium stores of the mitochondria and of the endoplasmic reticulum through phospholipase C activation. The inhibition of the calcium sensing receptor with 10 µM NPS-2143 prevented the L-C dependent cytosolic calcium increase suggesting that this calcium mobilization is mediated by Calcium Sensing Receptor activation. These results unravel that L-C might be part of a complex nutritional environment which tunes osteoblast activity.