Identification and functional characterization, including cytokine production modulation, of the novel chicken Interleukin-11. (October 2018)

• Record Type: Journal Article
• Authors: Truong, Anh Duc; Hong, Yeojin; Rengaraj, Deivendran; Lee, Janggeun; Lee, Kyungbaek; Hong, Yeong Ho
• Is Part Of: Developmental and comparative immunology. Volume 87(2018)
• Page Start: 51
• Page End: 63
• Publication Date: 2018-10
• Subjects: Interleukin-11 -- Anti-inflammatory -- Chicken -- JAK/STAT -- NF-κB -- Pathways; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2018.05.017
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interleukin (IL)-11 plays an important role in the immune system. However, IL-11 has not yet been characterized in avian species, including chickens. This study is the first to clone and functionally characterize chicken IL-11 (chIL-11). Multiple alignments and phylogenetic tree comparisons of chIL-11 with IL-11 proteins from other species revealed high levels of conservation and a close relationship between chicken and Japanese quail IL-11. Our results demonstrate that chIL-11 was a functional ligand of IL-11RA and IL-6ST in chicken HD11 and OU2 cell lines, as well as activated and regulated JAK-STAT, NF-κB, PI3K/AKT, and MAPK signaling pathways in chicken cell lines. In addition, chIL-11 inhibited nitric oxide production, affected proliferation of both tested cell lines, inhibited Type 1 and 17 T helper (Th) cytokine and IL-26, IL-12, and IL-17A-induced interferon-γ production, and enhanced Th2 cytokine (IL-4 and IL-10) production. Taken together, functional analysis of chIL-11 revealed it bound to IL-11RA and IL-6ST and activated the JAK-STAT, NF-κB, and MAPK signaling pathways, which resulted in modulation of Th1/Th17 and Th2 cytokine production in chicken HD11 and OU2 cell lines. Overall, this indicates chIL-11 has a role in both the innate and adaptive immune system. Highlights: Cloning and sequence analysis of chicken interleukin 11 (chIL-11). chIL-11 has an anti-inflammatory related to IL-11RA and IL-6ST receptor expression. chIL-11 activated and regulated JAK-STAT, NF-κB, PI3K/AKT, and MAPK signaling pathways. chIL-11 inhibited nitric oxide production, affected proliferation of both tested cell lines. chIL-11 inhibited Th1, Th17 cytokine and enhanced Th2 cytokine production.