PcLys-i3, an invertebrate lysozyme, is involved in the antibacterial immunity of the red swamp crayfish, Procambarus clarkii. (October 2018)

• Record Type: Journal Article
• Authors: Liu, Yan; Zhang, Ying-Hao; Li, Tong; Cao, Xiao-Tong; Zhou, Yang; Yuan, Jun-Fa; Gu, Ze-Mao; Lan, Jiang-Feng
• Is Part Of: Developmental and comparative immunology. Volume 87(2018)
• Page Start: 109
• Page End: 115
• Publication Date: 2018-10
• Subjects: Procambarus clarkii -- Lysozymes -- Innate immunity -- Vibrio parahaemolyticus; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2018.05.020
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Antimicrobial peptides (AMPs) play important roles in innate immunity against pathogens and lysozymes are a particularly type of AMP. Lysozymes are hydrolytic enzymes that are characterized by their ability to cleave the beta-(1, 4)-glycosidic bond between N -acetylmuramic acid and N -acetylglucosamine in peptidoglycan, which is the major bacterial cell wall polymer. In this work, a lysozyme was identified from Procambarus clarkii and designated PcLys-i3 . Quantitative RT-PCR was used to analyze the tissue distribution and expression profiles of PcLys-i3 . PcLys-i3 was present in all tested tissues and had high expression levels in gills, stomach and intestine. The expression levels of PcLys-i3 were up-regulated in gills and intestine after challenge with Vibrio parahaemolyticus, Staphylococcus aureus and Aeromonas hydrophila . PcLys-i3 and PcFer proteins can enhance the bacterial elimination in crayfish, whereas the bacterial elimination was weakened when the expression level of PcLys-i3 or PcFer RNAs was suppressed by RNAi. Recombinant PcLys-i3 and PcFer significantly reduced the mortality of crayfish with bacterial infections. Further study found that PcLys-i3 could interact with PcFer in vitro . Finally, the PcLys-i3 and PcFer proteins could bind to bacteria and inhibit bacterial replication. These results suggest that both PcLys-i3 and PcFer play important roles in the antibacterial immunity of red swamp crayfish. Highlights: PcLys-i3 was expressed in several tissues. The expression level of PcLys-i3 was up-regulated expression after challenge with bacteria. PcLys-i3 and PcFer proteins can enhance the elimination of bacteria in crayfish. PcLys-i3 may interact with PcFer.