The combination of Puerariae Lobatae Radix and Chuanxiong Rhizoma enhanced the absorption and pharmacokinetics of puerarin by modulating the intestinal barrier and influenced gut microbiota. (August 2018)

• Record Type: Journal Article
• Authors: Chen, Runzhi; Wu, Peng; Cai, Zheng; Tang, Lan; Ye, Ling; Hou, Chuqi; Yang, Na; Zhao, Jie
• Is Part Of: Journal of functional foods. Volume 47(2018)
• Page Start: 72
• Page End: 82
• Publication Date: 2018-08
• Subjects: Puerarin -- Ligustrazine -- Absorption -- Pharmacokinetics -- Intestinal barrier -- Gut microbiota; Puerarin (PubChem CID: 5281807) -- Daidzein (PubChem CID: 5281708) -- Daidzin (PubChem CID: 107971) -- Ligustrazine (PubChem CID: 14296); PLR Puerariae Lobatae Radix -- CXR Chuanxiong Rhizoma -- CVD cardiovascular diseases -- P-gp P-glycoprotein -- LDL low density lipoprotein -- HDL high density lipoprotein -- TG triglyceride -- T-CHO total cholesterol -- DAO diamine oxidase -- LPS lipopolysaccharide -- SPIP in situ single-pass intestinal perfusion -- Peff effective permeability coefficient -- Pw intestinal wall permeability -- PCA principal component analysis -- PCoA principal coordinate analysis -- NMDS nonmetric multidimensional scaling -- FDR false discovery rate -- LEfSe linear discriminant analysis effect size -- LDA linear discriminant analysis; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.05.043
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Daidzin and daidzein enhanced the intestinal absorption of puerarin. Ligustrazine improved the absorption of puerarin both in situ and in vivo. PLR with CXR increased intestinal permeability. PLR with CXR modulated P-gp and claudin-5 to affect intestinal barrier. PLR with CXR influenced gut microbiota, which may be a potential target. Abstract: Puerarin, the most important flavonoid in Puerariae Lobatae Radix (PLR), has high biological activity but poor oral absorption. This study investigated how to enhance the absorption and pharmacokinetics of puerarin through modulating the intestinal barrier. Analyses of intestinal absorption, pharmacokinetics demonstrated enhanced puerarin absorption with daidzin, daidzein and ligustrazine (the main active ingredient in vinegar and Chuanxiong Rhizoma (CXR)). In particular, PLR combined with CXR enhanced the absorption and pharmacokinetics of puerarin through elevating puerarin solubility, regulating P-glycoprotein efflux and reducing claudin-5 expression. The combination of PLR and CXR reduced the diversity of gut microbiota and the ratio of Firmicutes to Bacteroidetes . In conclusion, combining PLR with CXR enhanced the absorption and pharmacokinetics of puerarin by modulating the intestinal barrier and influenced gut microbiota. The gut microbiota is likely to be a potential target for the combination of PLR and CXR.