Cultivation of Chlorella protothecoides under different growth modes and its utilisation in oil/water emulsions. (September 2019)

• Record Type: Journal Article
• Authors: Caporgno, Martín P.; Haberkorn, Iris; Böcker, Lukas; Mathys, Alexander
• Is Part Of: Bioresource technology. Volume 288(2019)
• Publication Date: 2019-09
• Subjects: Chlorella protothecoides -- Bio-based products -- Pigments -- Oil in water (O/W) emulsions -- Minimally processed biomass; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121476
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: The highest biomass productivity was achieved in mixotrophic growth. Reduced greenish colouration was observed in media supplemented with glucose. Stable O/W emulsions (50% of oil) were produced using whole microalgae fractions. Simplified microalgae downstream processing prior to preparing the emulsion. Abstract: Microalgae can be incorporated in different bio-based products; however, the green colour is a barrier for a successful integration. This study aims to overcome this barrier by growing microalgae in different cultivation modes. Mixotrophic cultivation of Chlorella protothecoides resulted in the highest biomass production after 5 days (5.56 ± 0.09 g/L), followed by heterotrophic and photoautotrophic cultivation (4.33 ± 0.15 and 1.80 ± 0.05 g/L, respectively). Mixotrophically and heterotrophically produced biomass presented a reduced greenish colouration compared to photoautotrophically produced biomass. Chlorophyll content resulted in 1.46 ± 0.21 and 0.95 ± 0.28 mg/g dry weight (DW) in mixotrophic and heterotrophic cultures, respectively, and 25.98 ± 1.28 mg/g DW in photoautotrophic cultures. In contrast, the fraction of carotenoids in the total pigments was much higher. With the whole microalgae fractions after cell disruption as ingredients, stable emulsions containing 50% oil could be produced. No syneresis with serum separation was observed 24 h after preparation.