Peripheral Gamma Delta T cells secrete inflammatory cytokines in women with idiopathic recurrent pregnancy loss. (February 2018)

• Record Type: Journal Article
• Authors: Talukdar, Ayantika; Rai, Reeta; Aparna Sharma, K.; Rao, D.N.; Sharma, Alpana
• Is Part Of: Cytokine. Volume 102(2018)
• Page Start: 117
• Page End: 122
• Publication Date: 2018-02
• Subjects: Gamma Delta (γδ) T cells -- IFN-γ: Interferon gamma -- IL-10: Interleukin 10 -- IL-17: Interleukin 17 -- Recurrent pregnancy loss -- TGF-β: Tumor Growth Factor-beta; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2017.07.018
• Languages: English
• ISSNs: 1043-4666

Abstract

Highlights: CD3 + CD4 − CD8 − γδ T cells are the major subset of γδ T cells in RPL. These cells are mainly involved in the production of IFNγ & IL17 in the circulation. Proportion of TGFβ producing CD3 + CD4 − CD8 − γδ T cells are less in RPL than in control. Enhanced IFN-γ and IL-17 by γδ T cells contributes towards inflammation in RPL. Abstract: Background: Gamma delta (γδ) T cells are known to link innate and adaptive immunity. Decidual γδ T cells are known to provide immunotolerance by producing IL-10 and TGF-β. In recurrent pregnancy loss (RPL) females, the role of peripheral γδ T cells remain unstudied. Objective: To investigate the different phenotypes of γδ T cells in the peripheral blood of women with idiopathic RPL and their possible involvement in RPL condition. Methods: A total of 120 women were recruited for the study. Peripheral blood lymphocytes were isolated and they were stained with appropriate antibodies to determine the phenotype of γδ T cells and major cytokines produced by them in the blood using flow cytometry. Results: We observed a significant decrease in the proportion of CD3 + CD4 − CD8 − γδ T cells (p < 0.001) and increase in the percentage of IFN-γ (p < 0.05) and IL-17 (p < 0.001) producing γδ T cells in RPL pregnant as compared to normal pregnant females. Conclusion: Increase in IFN-γ and IL-17-producing CD3 + CD4 − CD8 − γδ T cells is associated with creating inflammatory cytokine milieu, thereby, may contribute towards pregnancy loss in RPL females.