The use of microfabrication technology to address the challenges of building physiologically relevant vasculature. (June 2018)

• Record Type: Journal Article
• Authors: Bannerman, A. Dawn; Ze Lu, Rick Xing; Korolj, Anastasia; Kim, Lucie H.; Radisic, Milica
• Is Part Of: Current opinion in biomedical engineering. Volume 6(2018)
• Page Start: 8
• Page End: 16
• Publication Date: 2018-06
• Subjects: DAPI 4′, 6-diamidino-2-phenylindole -- B4F biotin-4-fluorescein -- CM cardiomyocyte -- EC endothelial cell -- ECM extracellular matrix -- GF growth factor -- HUVECs human umbilical vein endothelial cells -- MSCs mesenchymal stem cells -- microECP microelectronic cardiac patch -- MMP multi-photon photolithography -- 3D three-dimensional -- Tβ4 thymosin-β4 -- VEGF vascular endothelial growth factor; Microfabrication -- Vasculature -- Growth factors -- Drug testing -- Tissue engineering -- Cardiac tissue; Biomedical engineering -- Periodicals; 610.28
• DOI: 10.1016/j.cobme.2017.12.003
• Languages: English
• ISSNs: 2468-4511

Abstract

Abstract: Vasculature is an essential component of physiologically relevant tissue, yet mimicking the native vasculature is an ongoing challenge in tissue engineering. Microfabrication may provide a solution. This technique allows for control of material design and architecture at the micron scale, which in turn enables the incorporation of microscale cues that are important for cell culture and the encouragement of organized vascularization. This review discusses the use of microfabrication to control the local biochemical environments for cells, the biophysical stimuli to which cells respond, and the assembly of more realistic spatial arrangements of various cell and tissue types. Furthermore, we describe ways in which microfabrication techniques are being used to address some of the challenges in translating these engineered tissue platforms into clinically relevant systems. Highlights: Microfabrication generates features that control stimuli impacting vascularization. Local biochemical environments can be controlled to encourage angiogenesis. Biophysical cues such as topography guide cell behaviour through contact guidance. Precise spatial arrangement of various cell types can result in cell–cell crosstalk. Microfabrication helps with in vivo translation of vascularized engineered tissue.