Microbial community evolution and fate of antibiotic resistance genes during sludge treatment in two full-scale anaerobic digestion plants with thermal hydrolysis pretreatment. (September 2019)

• Record Type: Journal Article
• Authors: Tong, Juan; Fang, Ping; Zhang, Junya; Wei, Yuansong; Su, Yanyan; Zhang, Yifeng
• Is Part Of: Bioresource technology. Volume 288(2019)
• Publication Date: 2019-09
• Subjects: Sludge thermal hydrolysis -- Anaerobic digestion -- Microbial community -- Antibiotic resistance genes -- Multiple linear regression analysis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121575
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Dominant bacteria shifted from nutrients removal bacteria to fermentative bacteria. Hydrogenotrophic pathway is the main biochemical pathway of methanogenesis in AD. Full-scale thermal hydrolysis & AD selected erm B, erm F, mef A/E, qnr S and tet M. Archaea biomass and community presented important impacts on the fate of ARGs. Total ARGs in the sludge are mainly affected by MGEs during the advanced AD. Abstract: Anaerobic digestion (AD) with thermal hydrolysis pretreatment is widely used as an efficient sludge treatment nowadays. However, the evolution of microbial community (especially for the archaea community), the fate of antibiotic resistance genes (ARGs), and their associations during such process in full-scale sludge treatment plants are rarely reported. Therefore, these scientific questions were explored at two full-scale sludge treatment plants through high-throughput sequencing and quantitative PCR. Results showed that Methanobacterium and Methanosphaera were the dominant archaea in thermal hydrolyzed sludge. The predominant bacteria in the sludge first shifted from nutrients removal functional bacteria to spore-forming bacteria after thermal hydrolysis, and then shifted to fermentative bacteria after AD. The full-scale plants could select erm B, erm F, mef A/E, qnr S and tet M. Though the bacteria and archaea biomass and community largely influenced the fate of ARGs, multiple linear regression analysis showed that the total ARGs were mainly affected by mobile genetic elements (MGEs).