Enhanced growth-driven stepwise inducible expression system development in haloalkaliphilic desulfurizing Thioalkalivibrio versutus. (September 2019)

• Record Type: Journal Article
• Authors: Sharshar, Moustafa Mohamed; Samak, Nadia Abdrabo; Hao, Xuemi; Mu, Tingzhen; Zhong, Wei; Yang, Maohua; Peh, Sumit; Ambreen, Sadaf; Xing, Jianmin
• Is Part Of: Bioresource technology. Volume 288(2019)
• Publication Date: 2019-09
• Subjects: Thioalkalivibrio versutus -- Fed-batch cultivation -- Bio-desulfurization -- Inducible regulation system -- Promoter engineering; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.121486
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Fed-batch culturing strategy was studied in Thiolakalivibro versutus D306. Maximal growth rate and biomass production were improved by 1.6 fold and 3-fold, respectively. Preferred desulfurization product, sulfur, reached 49% in yield percent and 30 µm in diameter. Inducible regulation system was built using fed-batch coupled with DBTV approach. MazF toxin was expressed successfully from the inducible regulation system built. Abstract: Highly toxic and flammable H2 S gas has become an environmental threat. Because of its ability to efficiently remove H2 S by oxidation, Thioalkalivibrio versutus is gaining more attention. Haloalkaliphilic autotrophs, like the bio-desulfurizing T. versutus, grow weakly. Weak growth makes any trial for developing potent genetic tools required for genetic engineering far from achieved. In this study, the fed-batch strategy improved T. versutus growth by 1.6 fold in maximal growth rate, 9-fold in O.D600 values and about 3-fold in biomass and protein productions. The strategy also increased the favorable desulfurization product, sulfur, by 2.7 fold in percent yield and 1.5-fold in diameter. A tight iron-inducible expression system for T. versutus was successfully developed. The system was derived from fed-batch cultivation coupled with new design, build, test and validate (DPTV) approach. The inducible system was validated by toxin expression. Fed-batch cultivation coupled with DPTV approach could be applied to other autotrophs.