Transport of gallocatechin gallate and catechin gallate in high-temperature-processed green tea extract from gastrointestinal tract to brain by an in vitro bio-mimic model system coupled with sequential cell cultures. (August 2018)

• Record Type: Journal Article
• Authors: Jeong, Kang-Hyun; Cho, Si-Young; Hong, Yong-Deog; Chung, Jin-Oh; Kim, Kwang-Sik; Shim, Soon-Mi
• Is Part Of: Journal of functional foods. Volume 47(2018)
• Page Start: 83
• Page End: 90
• Publication Date: 2018-08
• Subjects: GCG -- CG -- Metabolite -- In vitro bio-mimic model; BBB blood-brain barrier -- CG catechin gallate -- GCG gallocatechin gallate -- GI gastrointestinal -- HBMECs human brain microvascular endothelial cells -- HTP_GTE high-temperature-processed green tea extract; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.05.028
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: GCG and CG transported into BBB with high permeability at low concentration. Bio-accessibility and BBB transport of both catechins depend on their chemical structure. GCG and CG are metabolized as conjugated with glucuronide or sulfate. Abstract: The aim of this study was to evaluate the transport of gallocatechin gallate (GCG), catechin gallate (CG) and their metabolites in high-temperature-processed green tea extract (HTP_GTE) from the gastrointestinal tract to blood-brain barrier (BBB) with an in vitro bio-mimic model system with sequential cell cultures including Caco-2, HepG2 and HBMECs. Transports from the GI to BBB, the final concentrations of GCC and CG were 18.95 ± 3.24 μM and 5.11 ± 0.83 μM, respectively. Metabolites detected in BBB after systemic circulation were identified as glucuronide or sulfate conjugated form. Results suggest that GCG, CG and their metabolites in HTP_GTE are capable of reaching the brain by oral intake of HTP_GTE, implying that HTP_GTE could be utilized as a natural functional material for the prevention of degenerative brain diseases.