The diversity of venom components of the scorpion species Paravaejovis schwenkmeyeri (Scorpiones: Vaejovidae) revealed by transcriptome and proteome analyses. (1st September 2018)

• Record Type: Journal Article
• Authors: Cid-Uribe, Jimena I.; Santibáñez-López, Carlos E.; Meneses, Erika P.; Batista, Cesar V.F.; Jiménez-Vargas, Juana M.; Ortiz, Ernesto; Possani, Lourival D.
• Is Part Of: Toxicon. Volume 151(2018)
• Page Start: 47
• Page End: 62
• Publication Date: 2018-09-01
• Subjects: Paravaejovis schwenkmeyeri -- Proteome -- Scorpion -- Transcriptome -- Venom; Toxins -- Periodicals; Venom -- Periodicals; 615.9
• DOI: 10.1016/j.toxicon.2018.06.085
• Languages: English
• ISSNs: 0041-0101

Abstract

Abstract: The recent publication of high-throughput transcriptomic and proteomic analyses of scorpion venom glands has increased our knowledge on the biodiversity of venom components. In this contribution, we report the transcriptome of the venom gland and the proteome of the venom for the scorpion species Paravaejovis schwenkmeyeri, a member of the family Vaejovidae. We report 138 annotated transcripts encoding putative peptides/proteins with sequence identity to known venom components available from different databases. A fingerprint analysis containing the molecular masses of 212 components of the whole soluble venom revealed molecular weights of approximately 700 to 13, 800 Da, with most detected proteins ranging from 1500 to 3000 Da. Amino acid sequencing of venom components by LC-MS/MS allowed the identification of fragments from 27 peptides encoded by transcripts found in the transcriptome analysis. Enzymatic assays conducted with the soluble venom fraction confirmed the presence of enzymes such as hyaluronidases and phospholipases. The database presented here increases our general knowledge on the biodiversity of venom components from neglected non-buthid scorpions. Highlights: The transcriptome of the venom gland from Paravaejovis schwenkmeyeri was sequenced. A complete proteome analysis of the Paravaejovis schwenkmeyeri venom was performed. High-throughput RNAseq and LC-MS/MS technologies were employed. A total of 138 transcripts encoding putative venom peptides were annotated. The presence in the venom of the peptides encoded by 27 transcripts was confirmed.