Microbial Production of l-Serine from Renewable Feedstocks. Issue 7 (July 2018)

• Record Type: Journal Article
• Authors: Zhang, Xiaomei; Xu, Guoqiang; Shi, Jinsong; Koffas, Mattheos A.G.; Xu, Zhenghong
• Is Part Of: Trends in biotechnology. Volume 36:Issue 7(2018)
• Page Start: 700
• Page End: 712
• Publication Date: 2018-07
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Genetic engineering -- Periodicals; Industrial microbiology -- Periodicals; 660.605
• DOI: 10.1016/j.tibtech.2018.02.001
• Languages: English
• ISSNs: 0167-7799

Abstract

Abstract : l -Serine is a non-essential amino acid that has wide and expanding applications in industry with a fast-growing market demand. Currently, extraction and enzymatic catalysis are the main processes for l -serine production. However, such approaches limit the industrial-scale applications of this important amino acid. Therefore, shifting to the direct fermentative production of l -serine from renewable feedstocks has attracted increasing attention. This review details the current status of microbial production of l -serine from renewable feedstocks. We also summarize the current trends in metabolic engineering strategies and techniques for the typical industrial organisms Corynebacterium glutamicum and Escherichia coli that have been developed to address and overcome major challenges in the l -serine production process. Highlights: Identifying exporters for l -serine can reduce l -serine feedback inhibition and therefore enhance l -serine production. High-throughput screening using biosensors can be developed for l -serine and used for strain engineering. The split between l -serine consumption for cell growth and extracellular l -serine accumulation can be balanced by tunable control of metabolic flows combined with 13 C metabolic flux analysis. Systems biology methods, including transcriptomics, proteomics, and metabolomics analysis, can be used for identifying targets for optimizing l -serine production. Genome-scale metabolic models can prove to be useful for systematically identifying genetic targets for improving l -serine production. To reduce the cost for commercializing l -serine production, it is essential to explore alternative and cheaper carbon sources.