A potential efflux boron transporter gene GsBOR2, positively regulates Arabidopsis bicarbonate tolerance. (September 2018)

• Record Type: Journal Article
• Authors: Duan, Xiangbo; Yu, Yang; Zhang, Yu; Chen, Chao; Duanmu, Huizi; Cao, Lei; Sun, Mingzhe; Sun, Xiaoli; Zhu, Yanming
• Is Part Of: Plant science. Volume 274(2018)
• Page Start: 284
• Page End: 292
• Publication Date: 2018-09
• Subjects: A. thaliana Arabidopsis thaliana -- BOR high boron requiring -- G. max Glycine max -- G. soja Glycine soja -- LiAc lithium acetate -- M. truncatula Medicago truncatula -- MDA malondialdehyde -- MES 2- (N-Morpholino) ethanesulfonic acid -- MS medium Murashige and Skoog medium -- O. sativa Oryza sativa -- OX overexpression -- P. patens Physcomitrella patens -- PEG polyethylene glycol -- S. moellendorffii Selaginella moellendorffii -- SD medium synthetic dropout medium -- SLC4 solute carrier family 4 -- T-DNA transfer DNA -- Ura uracil -- WT wild type -- Z. mays Zea mays; Glycine soja -- Efflux transporter -- Boron -- Alkaline stress -- Bicarbonate; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2018.05.032
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: The expression pattern of GsBOR2 was analyzed. Overexpression of GsBOR2 enhanced alkaline tolerance. Overexpression of GsBOR2 specifically enhanced bicarbonate tolerance. GsBOR2 is a potential boron exporter that mediates boron efflux from cells. Abstract: Soil alkalization severely restricts agricultural production and economic development worldwide, this problem is far more serious in Songnen Plain, the largest commodity grain base of China. However, little research has been done concerning the mechanisms of plant responses to alkaline stress to date. In this study, we isolated an alkali inducible gene GsBOR2 from Glycine soja on the basis of RNA seq data. GsBOR2 sh high protein sequence similarity with the known boron transporters in other species. The expression of GsBOR2 was highly up-regulated by 50 mM NaHCO3 treatment and displayed tissue specificity. We then generated the transgenic Arabidopsis overexpressing GsBOR2 and found that the transgenic lines exhibited enhanced alkaline tolerance compared to wild type plants, as illustrated by longer roots and greater shoot biomass. Moreover, GsBOR2 overexpression was also capable of increasing plant resistance to KHCO3 treatment but not to high-pH stress. Functional complementation of Scbor1 mutant yeasts suggested that GsBOR2 could likely mediate the efflux of boron from cells. Taken together, the alkali responsive gene GsBOR2 is a positive regulator of plant bicarbonate tolerance.