Enzymatic/Immunoassay Dual‐Biomarker Sensing Chip: Towards Decentralized Insulin/Glucose Detection. (3rd April 2019)

• Record Type: Journal Article
• Authors: Vargas, Eva; Teymourian, Hazhir; Tehrani, Farshad; Eksin, Ece; Sánchez‐Tirado, Esther; Warren, Paul; Erdem, Arzum; Dassau, Eyal; Wang, Joseph
• Is Part Of: Angewandte Chemie. Volume 131:Number 19(2019)
• Page Start: 6442
• Page End: 6445
• Publication Date: 2019-04-03
• Subjects: Biosensoren -- Detektierung dualer Marker -- Diabetes-Monitoring -- Elektrochemie -- Immunoassays; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201902664
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Performing bioassay formats based on enzyme and antibody recognition reactions with a single detection chip remains an unmet challenge owing to the different requirements of such bioassays. Herein, we describe a dual‐marker biosensor chip, integrating enzyme and antibody‐based assays for simultaneous electrochemical measurements of insulin (I) and glucose (G). Simultaneous G/I sensing has been realized by addressing key fabrication and operational challenges associated with the different assay requirements and surface chemistry. The I immunosensor relies on a peroxidase‐labeled sandwich immunoassay, while G is monitored through reaction with glucose oxidase. The dual diabetes biomarker chip offers selective and reproducible detection of picomolar I and millimolar G concentrations in a single microliter sample droplet within less than 30 min, including direct measurements in whole blood and saliva samples. The resulting integrated enzymatic‐immunoassay biosensor chip opens a new realm in point‐of‐care multiplexed biomarker detection. Abstract : Ein Dual‐Marker‐Biosensorchip, der Enzym‐ und Antikörper‐basierte Assays für die gleichzeitige elektrochemische Messung von Insulin (I) und Glucose (G) integriert, wird vorgestellt. Die gleichzeitige G/I‐Messung wurde umgesetzt, indem Schlüsselprobleme in Herstellung und Betrieb, die mit verschiedenen Assay‐Anforderungen und Oberflächenchemie verknüpft sind, angegangen wurden.