Uncovering the In Vivo Proxisome Using Proximity‐Tagging Methods. (30th October 2019)

• Record Type: Journal Article
• Authors: Santin, Yoann G.
• Is Part Of: BioEssays. Volume 41:Number 12(2019:Dec.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-10-30
• Subjects: APEX‐proximity‐dependent biotin labeling -- bacteria -- biotin identification -- in vivo proximity labeling -- protein–protein interaction -- proxisome -- pupylation‐based interaction tagging; Molecular biology -- Periodicals; Cytology -- Periodicals; Developmental biology -- Periodicals; 572.8
• DOI: 10.1002/bies.201900131
• Languages: English
• ISSNs: 0265-9247

Abstract

Abstract: The development of new approaches is critical to gain further insights into biological processes that cannot be obtained by existing methods or technologies. The detection of protein–protein interaction is often challenging, especially for weak and transient interactions or for membrane proteins. Over the last decade, several proximity‐tagging methodologies have been developed to explore protein interactions in living cells. Among those, the most efficient are based on protein partner modification, such as biotinylation or pupylation. Such technologies are based on engineered variants of enzymes like peroxidases or ligases that release reactive molecules, in the presence of specific substrates, that bind surrounding proteins. Fusing a protein of interest (POI) to these enzymes allows the definition of an unbiased "proxisome, " that is, all of the proteins in interaction or in close vicinity of the POI. Here, the different proximity‐labeling tools available are described and comprehensive comparison to discuss advantages and limitations is provided. Abstract : In living cells, exploration of protein–protein interactions is challenging. Recently, proximity‐tagging methods have been developed to overcome classical limitations related to conventional methods. Here, a comprehensive comparison of the major proximity‐tagging methods to help defining the most suitable approach for the studies is provided.