A Cytoplasmic Protein Ssl3829 Is Important for NDH-1 Hydrophilic Arm Assembly in Synechocystis sp. Strain PCC 6803. Issue 2 (18th April 2016)

• Record Type: Journal Article
• Authors: Wang, Xiaozhuo; Gao, Fudan; Zhang, Jingsong; Zhao, Jiaohong; Ogawa, Teruo; Ma, Weimin
• Is Part Of: Plant physiology. Volume 171:Issue 2(2016)
• Page Start: 864
• Page End: 877
• Publication Date: 2016-04-18
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.01796
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : A 10-kD cytoplasmic protein, Ssl3829, plays an important role in NDH-1 assembly by accumulating subunit maturation factor and assembly intermediate in a cyanobacterium. Abstract: Despite significant progress in clarifying the subunit compositions and functions of the multiple NDH-1 complexes in cyanobacteria, the assembly factors and their roles in assembling these NDH-1 complexes remain elusive. Two mutants sensitive to high light for growth and impaired in NDH-1-dependent cyclic electron transport around photosystem I were isolated from Synechocystis sp. strain PCC 6803 transformed with a transposon-tagged library. Both mutants were tagged in the ssl3829 gene encoding an unknown protein, which shares significant similarity with Arabidopsis ( Arabidopsis thaliana ) CHLORORESPIRATORY REDUCTION7. The ssl3829 product was localized in the cytoplasm and associates with an NDH-1 hydrophilic arm assembly intermediate (NAI ) of about 300 kD (NAI300) and an NdhI maturation factor, Slr1097. Upon deletion of Ssl3829, the NAI300 complex was no longer visible on gels, thereby impeding the assembly of the NDH-1 hydrophilic arm. The deletion also abolished Slr1097 and consequently reduced the amount of mature NdhI in the cytoplasm, which repressed the dynamic assembly process of the NDH-1 hydrophilic arm because mature NdhI was essential to stabilize all functional NAI s. Therefore, Ssl3829 plays an important role in the assembly of the NDH-1 hydrophilic arm by accumulating the NAI300 complex and Slr1097 protein in the cytoplasm.