A DNA2 Homolog Is Required for DNA Damage Repair, Cell Cycle Regulation, and Meristem Maintenance in Plants  . Issue 1 (7th March 2016)

• Record Type: Journal Article
• Authors: Jia, Ning; Liu, Xiaomin; Gao, Hongbo
• Is Part Of: Plant physiology. Volume 171:Issue 1(2016)
• Page Start: 318
• Page End: 333
• Publication Date: 2016-03-07
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.16.00312
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : A DNA2 homolog plays an important role in DNA damage repair, cell cycle progression, and the development of the shoot apical meristem and root apical meristem in higher plants. Abstract: Plant meristem cells divide and differentiate in a spatially and temporally regulated manner, ultimately giving rise to organs. In this study, we isolated the Arabidopsis jing he sheng 1 ( jhs1 ) mutant, which exhibited retarded growth, an abnormal pattern of meristem cell division and differentiation, and morphological defects such as fasciation, an irregular arrangement of siliques, and short roots. We identified JHS1 as a homolog of human and yeast DNA Replication Helicase / Nuclease2, which is known to be involved in DNA replication and damage repair. JHS1 is strongly expressed in the meristem of Arabidopsis. The jhs1 mutant was sensitive to DNA damage stress and had an increased DNA damage response, including increased expression of genes involved in DNA damage repair and cell cycle regulation, and a higher frequency of homologous recombination. In the meristem of the mutant plants, cell cycle progression was delayed at the G2 or late S phase and genes essential for meristem maintenance were misregulated. These results suggest that JHS1 plays an important role in DNA replication and damage repair, meristem maintenance, and development in plants.