Environmentally relevant concentrations of arsenite induces developmental toxicity and oxidative responses in the early life stage of zebrafish. (November 2019)

• Record Type: Journal Article
• Authors: Sun, Hong-Jie; Zhang, Jing-Ying; Wang, Qiang; Zhu, Engao; Chen, Wenrong; Lin, Hongjun; Chen, Jianrong; Hong, Huachang
• Is Part Of: Environmental pollution. Volume 254(2019)Part A
• Publication Date: 2019-11
• Subjects: Arsenite -- Low concentration -- Zebrafish -- Early life stage -- Oxidative stress; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.113022
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Arsenic (As) present in water is a nonignorable environmental issue, even at low concentrations (≤150 μg L −1 ). To evaluate the toxic effect of low concentrations of As, zebrafish at early life stage were exposed to 0, 25, 50, 75, or 150 μg L −1 AsIII for 120 h. Our results indicated that low concentration of AsIII decreased zebrafish larvae's survival rate to 85%, 89% and 86% at 50, 75 and 150 μg L −1 . Furthermore, low concentrations of AsIII exposure caused oxidative stress (elevated superoxide dismutase (SOD) activity and influenced the mRNA transcriptional levels of Cu/ZnSOD and MnSOD) and damage (increased malondialdehyde levels). Meanwhile, zebrafish larvae regulated the mRNA transcription of metallothionein and heat shock protein 70 to alleviate toxicity caused by AsIII. These results revealed lower concentrations (≤150 μg L −1 ) of AsIII had a detriment effect on the survival of fish at early life stage, moreover, oxidative stress caused by AsIII posed potential risk for the zebrafish. This study provides novel insight into low concentration AsIII-induced toxicity in zebrafish. Graphical abstract: Image 10447 Highlights: Our study focused on the toxic effect of low concentrations of arsenite on the zebrafish at early life stage. Low arsenite concentration decreased the survival rate of zebrafish at early life stage. Low arsenite concentration caused oxidative stress and damage on zebrafish at early life stage. Low arsenite concentration disturbed MT and HSP70 mRNA transcriptional levels of zebrafish at early life stage. Abstract : Arsenite caused oxidative stress and damage to zebrafish at early life stage.