A Predictive Coexpression Network Identifies Novel Genes Controlling the Seed-to-Seedling Phase Transition in Arabidopsis thaliana    . Issue 4 (17th February 2016)

• Record Type: Journal Article
• Authors: Silva, Anderson Tadeu; Ribone, Pamela A.; Chan, Raquel L.; Ligterink, Wilco; Hilhorst, Henk W.M.
• Is Part Of: Plant physiology. Volume 170:Issue 4(2016)
• Page Start: 2218
• Page End: 2231
• Publication Date: 2016-02-17
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.01704
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : A seed-to-seedling gene coexpression network reveals homeodomain leucine zipper I transcription factor AtHB13 as a novel key regulator affecting primary root length during late seedling establishment. Abstract: The transition from a quiescent dry seed to an actively growing photoautotrophic seedling is a complex and crucial trait for plant propagation. This study provides a detailed description of global gene expression in seven successive developmental stages of seedling establishment in Arabidopsis ( Arabidopsis thaliana ). Using the transcriptome signature from these developmental stages, we obtained a coexpression gene network that highlights interactions between known regulators of the seed-to-seedling transition and predicts the functions of uncharacterized genes in seedling establishment. The coexpressed gene data sets together with the transcriptional module indicate biological functions related to seedling establishment. Characterization of the homeodomain leucine zipper I transcription factor AtHB13, which is expressed during the seed-to-seedling transition, demonstrated that this gene regulates some of the network nodes and affects late seedling establishment. Knockout mutants for athb13 showed increased primary root length as compared with wild-type (Columbia-0) seedlings, suggesting that this transcription factor is a negative regulator of early root growth, possibly repressing cell division and/or cell elongation or the length of time that cells elongate. The signal transduction pathways present during the early phases of the seed-to-seedling transition anticipate the control of important events for a vigorous seedling, such as root growth. This study demonstrates that a gene coexpression network together with transcriptional modules can provide insights that are not derived from comparative transcript profiling alone.