Guard Cells Integrate Light and Temperature Signals to Control Stomatal Aperture  . Issue 3 (16th January 2020)

• Record Type: Journal Article
• Authors: Kostaki, Kalliopi-Ioanna; Coupel-Ledru, Aude; Bonnell, Verity C.; Gustavsson, Mathilda; Sun, Peng; McLaughlin, Fiona J.; Fraser, Donald P.; McLachlan, Deirdre H.; Hetherington, Alistair M.; Dodd, Antony N.; Franklin, Keara A.
• Is Part Of: Plant physiology. Volume 182:Issue 3(2020)
• Page Start: 1404
• Page End: 1419
• Publication Date: 2020-01-16
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.19.01528
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : High temperature-mediated stomatal opening involves cross talk with light signaling pathways. Abstract: High temperature promotes guard cell expansion, which opens stomatal pores to facilitate leaf cooling. How the high-temperature signal is perceived and transmitted to regulate stomatal aperture is, however, unknown. Here, we used a reverse-genetics approach to understand high temperature-mediated stomatal opening in Arabidopsis ( Arabidopsis thaliana ). Our findings reveal that high temperature-induced guard cell movement requires components involved in blue light-mediated stomatal opening, suggesting cross talk between light and temperature signaling pathways. The molecular players involved include phototropin photoreceptors, plasma membrane H + -ATPases, and multiple members of the 14-3-3 protein family. We further show that phototropin-deficient mutants display impaired rosette evapotranspiration and leaf cooling at high temperatures. Blocking the interaction of 14-3-3 proteins with their client proteins severely impairs high temperature-induced stomatal opening but has no effect on the induction of heat-sensitive guard cell transcripts, supporting the existence of an additional intracellular high-temperature response pathway in plants.