ZnSe:Mn/ZnS quantum dots for the detection of microcystin by room temperature phosphorescence immunoassay. (24th July 2019)

• Record Type: Journal Article
• Authors: You, Jiaqi; Ma, Long; He, Yu; Ge, Yili; Song, Gongwu; Zhou, Jiangang
• Is Part Of: Micro & nano letters. Volume 14:Number 8(2019)
• Page Start: 892
• Page End: 896
• Publication Date: 2019-07-24
• Subjects: zinc compounds -- manganese -- II‐VI semiconductors -- chemical sensors -- biological techniques -- semiconductor quantum dots -- phosphorescence -- wide band gap semiconductors -- assembling -- regression analysis; quantum dot quenching -- microcystin detection -- assembling -- QD -- 1‐ethyl‐3‐(3‐dimethyllaminopropyl) carbodiimide‐N‐Hydroxysuccinimide chemistry -- monoclonal antimicrocystin‐Leucine Arginine antibody -- RTP immunoassay probe -- room temperature phosphorescence immunoassay probe -- antiMC‐LR antibody detection -- regression equation -- interference -- autofluorescence -- light scattering -- temperature 293 K to 298 K -- ZnSe:Mn‐ZnS; Nanotechnology -- Periodicals; Nanostructures -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1049/mnl.2018.5690
• Languages: English
• ISSNs: 1750-0443

Abstract

Abstract : This work has assembled ZnSe:Mn/ZnS quantum dots (QDs)‐monoclonal anti‐microcystin‐Leucine Arginine (anti‐MC‐LR) antibody bioconjugates as a room temperature phosphorescence (RTP) probe using 1‐ethyl‐3‐(3‐dimethyllaminopropyl) carbodiimide/N‐Hydroxysuccinimide chemistry for detecting MC‐LR. Once the specific interactions occurred between these MCs and their antibodies on the surface of ZnSe:Mn/ZnS QDs, the RTP of ZnSe:Mn/ZnS QDs was quenched with the increased concentration of MC‐LR. Under the optimised conditions, the linear range of MC‐LR was determined to be 0.9–8.8 nM. The linear relationship between ( P 0 – P )/ P 0 and concentration of MC‐LR were observed with the regression equation ( P 0 – P )/ P 0 = 0.03908C + 0.0456 with the detection limit of 0.038 nM. The developed RTP immunoassay favoured environmental applications since the interference from autofluorescence and scattering light was greatly eliminated.