Analysis of human saliva metabolome by direct immersion solid-phase microextraction LC and benchtop orbitrap MS. (April 2013)

• Record Type: Journal Article
• Authors: Bessonneau, Vincent; Bojko, Barbara; Pawliszyn, Janusz
• Is Part Of: Bioanalysis. Volume 5:Number 7(2013)
• Page Start: 783
• Page End: 792
• Publication Date: 2013-04
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.13.35
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: Saliva samples collected from one 58 year old male and one 35 year old female during 7 days of fasting were analyzed by direct immersion of both C18 and mixed-mode biocompatible solid-phase microextraction fibers, in combination with a LC–MS method using a benchtop orbitrap instrument in both positive and negative ionization modes, in order to evaluate the difference in terms of metabolite coverage.Results: The mixed-mode coating provided better results, with the simultaneous extraction of a higher number of both hydrophilic and hydrophobic metabolites. The ability of detected features to distinguish differences between the individuals and changes in saliva metabolome induced by diet was also demonstrated.Conclusion: Saliva may be useful for medical diagnostics as it is non-invasive. The use of biocompatible solid-phase microextraction fibers can play an important role as an alternative sample preparation method for untargeted LC–MS metabolomics studies on human saliva because it provides simultaneous extraction of metabolites with a wide range of polarity, thus allowing the detection of changes in metabolic pathways with unsupervised statistical analyses.