Novel technical developments in mass spectrometry imaging in 2020: A mini review. Issue 3 (16th March 2021)

• Record Type: Journal Article
• Authors: Dilmetz, Brooke A.; Lee, Yea‐Rin; Condina, Mark R.; Briggs, Matthew; Young, Clifford; Desire, Christopher T.; Klingler‐Hoffmann, Manuela; Hoffmann, Peter
• Other Names: Eeltink Sebastiaan guestEditor.
• Is Part Of: Analytical Science Advances. Volume 2:Issue 3/4(2021)
• Page Start: 225
• Page End: 237
• Publication Date: 2021-03-16
• Subjects: formalin‐fixed paraffin‐embedded -- MALDI -- mass spectrometry imaging -- reproducibility -- workflows; Chemistry, Analytic -- Periodicals; Science -- Periodicals; 543
• DOI: 10.1002/ansa.202000176
• Languages: English
• ISSNs: 2628-5452

Abstract

Abstract: The applicability of mass spectrometry imaging (MSI) has exponentially increased with the improvement of sample preparation, instrumentation (spatial resolution) and data analysis. The number of MSI publications listed in PubMed continues to grow with 378 published articles in 2020‐2021. Initially, MSI was just sensitive enough to identify molecular features correlating with distinct tissue regions, similar to the resolution achieved by visual inspection after standard immunohistochemical staining. Although the spatial resolution was limited compared with other imaging modalities, the molecular intensity mapping added a new exciting capability. Over the past decade, significant improvements in every step of the workflow and most importantly in instrumentation were made, which now enables the molecular analysis at a cellular and even subcellular level. Here, we summarize the latest developments in MSI, with a focus on the latest approaches for tissue‐based imaging described in 2020.