Toward improving the proteomic analysis of formalin-fixed, paraffin-embedded tissue. (August 2013)

• Record Type: Journal Article
• Authors: Fowler, Carol B; O'Leary, Timothy J; Mason, Jeffrey T
• Is Part Of: Expert review of proteomics. Volume 10:Number 4(2013)
• Page Start: 389
• Page End: 400
• Publication Date: 2013-08
• Subjects: antigen retrieval -- formalin-fixed, paraffin-embedded -- liquid chromatography-tandem mass spectrometry -- matrix-assisted, laser desorption/ionization-imaging mass spectrometry -- protein extraction -- proteomics -- reverse-phase protein array; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/14789450.2013.820531
• Languages: English
• ISSNs: 1478-9450

Abstract

Archival formalin-fixed, paraffin-embedded (FFPE) tissue and their associated diagnostic records represent an invaluable source of retrospective proteomic information on diseases for which the clinical outcome and response to treatment are known. However, analysis of archival FFPE tissues by high-throughput proteomic methods has been hindered by the adverse effects of formaldehyde fixation and subsequent tissue histology. This review examines recent methodological advances for extracting proteins from FFPE tissue suitable for proteomic analysis. These methods, based largely upon heat-induced antigen retrieval techniques borrowed from immunohistochemistry, allow at least a qualitative analysis of the proteome of FFPE archival tissues. The authors also discuss recent advances in the proteomic analysis of FFPE tissue; including liquid-chromatography tandem mass spectrometry, reverse phase protein microarrays and imaging mass spectrometry.