Differential regulation of IgA+ B cells in vitro by stromal cells from distinctive anatomical compartments. Issue 3 (21st December 2018)

• Record Type: Journal Article
• Authors: Cen, Selena Y.; Moreau, Joshua M.; Furlonger, Caren; Berger, Alexandra; Paige, Christopher J.
• Is Part Of: Journal of leukocyte biology. Volume 105:Issue 3(2019)
• Page Start: 507
• Page End: 518
• Publication Date: 2018-12-21
• Subjects: BM -- differentiation -- gut -- spleen; Leucocytes -- Periodicals; Reticulo-endothelial system -- Periodicals; 571.96
• DOI: 10.1002/JLB.1A0517-172RR
• Languages: English
• ISSNs: 0741-5400

Abstract

Abstract: B cell development is regulated by stromal cells (SCs) that form a supportive microenvironment. These SCs along with other cell types produce cytokines, chemokines, and adhesion molecules that guide B cell commitment and differentiation. BM, spleen (Sp), and the gut lamina propria (LP) constitute distinctive anatomical compartments that support B cell differentiation. In order to characterize and compare the signals necessary to generate IgA + B cells, we developed an in vitro system to co‐culture gut LP, BM, or Sp‐derived SCs with B lineage cells. Using this co‐culture system, we found that gut LP SCs promote IgA + B cell accumulation through the production of soluble stimulatory factors. In contrast to gut LP SCs, BM and splenic SCs were found to impair IgA + B cell accumulation in vitro. Taken together, these observations provide new insights into how SCs derived from different anatomical locations shape IgA + B cell responses. Abstract : Accumulation of IgA + B cells in vitro is enhanced by gut lamina propria stromal cells but inhibited by bone marrow and splenic stromal cells.