Kbtbd11 contributes to adipocyte homeostasis through the activation of upstream stimulatory factor 1. Issue 11 (November 2019)

• Record Type: Journal Article
• Authors: Watanabe, Kazuhisa; Yokota, Kazuha; Yoshida, Ken; Matsumoto, Ayumi; Iwamoto, Sadahiko
• Is Part Of: Heliyon. Volume 5:Issue 11(2019)
• Publication Date: 2019-11
• Subjects: Cell biology -- Cell differentiation -- Gene expression -- Gene regulation -- Transcription factor -- Biochemistry -- Molecular biology -- Obesity -- Kbtbd11 -- 3T3-L1 differentiation -- USF1 -- Transcriptional regulation; Research -- Periodicals; Medical sciences -- Periodicals; Natural history -- Periodicals; Social sciences -- Periodicals; Earth sciences -- Periodicals; Physical sciences -- Periodicals; 507.2
• DOI: 10.1016/j.heliyon.2019.e02777
• Languages: English
• ISSNs: 2405-8440

Abstract

Abstract: The present study aimed to investigate the transcriptional regulation of Kbtbd11 in adipose tissue. To elucidate the physiological role of Kbtbd11 gene expression, adipose Kbtbd11 mRNA expression levels were estimated under various feeding states in wild-type mice. Kbtbd11 expression increased in a time-dependent manner in the adipose tissue in mice fed on chow diet, whereas the promotion of Kbtbd11 mRNA expression by refeeding was attenuated in mice fed on high-fat (HF) diet, suggesting the suppression of Kbtbd11 mRNA expression under HF diets and that changes in mRNA levels were associated with regulation of the transcription activity of Kbtbd11 by some transcription factors. To investigate the transcriptional regulation of Kbtbd11, the fragment upstream of either mouse Kbtbd11 or human KBTBD11 promoter was inserted into a luciferase vector. Luciferase reporter assays revealed that both mouse and human KBTBD11 promoter activity was increased by USF1. Direct USF1 binding to the Ebox in the Kbtbd11 promoter was confirmed by electrophoretic mobility shift and chromatin immunoprecipitation assays. In addition, the adipocyte differentiation marker levels increased instantly in Kbtbd11 -overexpressing Usf1 knockdown cells than in Usf1 knockdown cells. These results imply an association of between Kbtbd11 with Usf1 expression and suggest the involvement of Kbtbd11 in a novel adipogenesis pathway. Abstract : Cell biology; Cell Differentiation; Gene Expression; Gene Regulation; Transcription Factor; Biochemistry; Molecular Biology; Obesity; Kbtbd11 ; 3T3-L1 differentiation; USF1; transcriptional regulation.