Employing metabolic engineered lipolytic microbial platform for 1-alkene one-step conversion. (September 2018)

• Record Type: Journal Article
• Authors: Wang, Juli; Yu, Haiying; Zhu, Kun
• Is Part Of: Bioresource technology. Volume 263(2018)
• Page Start: 172
• Page End: 179
• Publication Date: 2018-09
• Subjects: 1-Alkene -- Lipolytic microbe -- Biofuel -- Metabolic engineering -- Pseudomonas; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.04.119
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Lipolytic microbial platform shortened metabolic process for 1-alkene production. Integration of UndB into chromosome successfully led to 1-alkene production. Lauric acid mainly led to 1-undecene and palm oil mainly led to 1-pentadecene. Intracellular release of fatty acids by thioesterases enhanced 1-alkene production. Adjustments of fatty acid degradation pathway improved 1-alkene titer. Abstract: 1-Alkenes are traditionally used as basic chemicals with great importance. Biosynthetic 1-alkenes also have the potential to serve as biofuels. In this study, we engineered a Pseudomonas lipolytic microbial platform for 1-alkene production using hydrophobic substrate as sole carbon source. Fatty acid decarboxylase UndA and UndB were cloned and expressed, which successfully produced 1-alkenes. Optimal culturing temperature and the interruption of competitive pathway were proven to be beneficial to 1-alkene synthesis. Chromosomal integration of UndB conferred 177.8 mg/L 1-alkenes (mainly 1-undecene) in lauric acid medium and 128.9 mg/L 1-alkenes (mainly 1-pentadecene) in palm oil medium. Thioesterase expression, adjustments of fatty acid degradation pathway and a second copy of UndB improved 1-alkene titer to 1102.6 mg/L using lauric acid and 778.4 mg/L using palm oil. All in all, this study offers the first demonstration of lipolytic microbial 1-alkene producing platform with highest reported 1-alkene product titer up to date.