Creating biotransformation of volatile fatty acids and octanoate as co-substrate to high yield medium-chain-length polyhydroxyalkanoate. (July 2021)

• Record Type: Journal Article
• Authors: Li, Dongna; Ma, Xiaojun; Yin, Fen; Qiu, Yujuan; Yan, Xu
• Is Part Of: Bioresource technology. Volume 331(2021)
• Publication Date: 2021-07
• Subjects: Wood waste -- Mixed microbial consortia -- Polyhydroxyalkanoate -- Co-substrate -- Microbial Community; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.125031
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Mcl-PHA was produced using VFAs from waste wood and octanoate as co-substrates. High PHA production of 1834 mg COD/L and 3HO proportion of 22.5% were obtained. Co-substrate shortened the period of PHA reaching the maximum production. Mechanical properties of PHA were 11.73 ± 0.625 MPa and 1047.11 ± 30.72 MPa. Microbial community was composed of 46 bacterial genera with higher diversity. Abstract: Using mixed microbial consortium (MMC) to accumulate polyhydroxyalkanoate (PHA) is an effective strategy to solve high production cost and reduce the amount of excess sludge. In this study, a process for the production of short-chain-length and medium-chain-length PHA using volatile fatty acids (VFAs) from pretreated wood hydrolysate synergistic with octanoate as co-substrate was proposed. The effects of co-substrate ratios on PHA accumulation ability and physical properties were investigated. The incorporation of co-substrate accelerated the time of PHA and 3-hydroxyoctanoate reaching the maximum production (1834 and 280 mg COD/L). The highest PHA content was 53.0% (w/w), which was equivalent to that reported previously. The biopolymer films possessed high tensile strength, Young's modulus, and could be used in the field of water vapor barrier requirements. The accumulation strategy applied for converting fermentation products VFAs and octanoate co-substrate into high value and yield PHA could potentially demonstrate the valuable for low-cost large-scale production.