Proteomic and metabolomic responses in hepatopancreas of whiteleg shrimp Litopenaeus vannamei infected by microsporidian Enterocytozoon hepatopenaei. Issue 87 (April 2019)

• Record Type: Journal Article
• Authors: Ning, Mingxiao; Wei, Panpan; Shen, Hui; Wan, Xihe; Jin, Mingjian; Li, Xiangqian; Shi, Hao; Qiao, Yi; Jiang, Ge; Gu, Wei; Wang, Wen; Wang, Li; Meng, Qingguo
• Is Part Of: Fish & shellfish immunology. Issue 87(2019)
• Page Start: 534
• Page End: 545
• Publication Date: 2019-04
• Subjects: Enterocytozoon hepatopenaei -- Litopenaeus vannamei -- Hepatopancreatic microsporidiosis -- Proteomics -- Metabolomics; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.01.051
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Enterocytozoon hepatopenaei (EHP) causes hepatopancreatic microsporidiosis (HPM) in shrimp. HPM is not normally associated with shrimp mortality, but is associated with significant growth retardation. In this study, the responses induced by EHP were investigated in hepatopancreas of shrimp Litopenaeus vannamei using proteomics and metabolomics. Among differential proteins identified, several (e.g., peritrophin-44-like protein, alpha2 macroglobulin isoform 2, prophenoloxidase-activating enzymes, ferritin, Rab11A and cathepsin C) were related to pathogen infection and host immunity. Other proteomic biomarkers (i.e., farnesoic acid o -methyltransferase, juvenile hormone esterase-like carboxylesterase 1 and ecdysteroid-regulated protein) resulted in a growth hormone disorder that prevented the shrimp from molting. Both proteomic KEGG pathway (e.g., "Glycolysis/gluconeogenesis" and "Glyoxylate and dicarboxylate metabolism") and metabolomic KEGG pathway (e.g., "Galactose metabolism" and "Biosynthesis of unsaturated fatty acids") data indicated that energy metabolism pathway was down-regulated in the hepatopancreas when infected by EHP. More importantly, the changes of hormone regulation and energy metabolism could provide much-needed insight into the underlying mechanisms of stunted growth in shrimp after EHP infection. Altogether, this study demonstrated that proteomics and metabolomics could provide an insightful view into the effects of microsporidial infection in the shrimp L. vannamei . Graphical abstract: Image 1 Highlights: 266 differently expressed proteins and 49 differential metabolites were identified. 7 immunologic proteins were verified for their immune role by Real-time PCR. The change of growth associated proteins might prevent the shrimp from molting. The energy metabolism pathway was down-regulated in the shrimp hepatopancreas.