Pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity. Issue 87 (April 2019)

• Record Type: Journal Article
• Authors: Meng, Liang; Xu, Wen-teng; Chen, Ya-dong; Wei, Min; Cui, Zhong-kai; Liu, Yang; Guo, Hua; Gan, Xi; Zhu, Jia-jie; Wang, Lin-na; Chen, Song-lin
• Is Part Of: Fish & shellfish immunology. Issue 87(2019)
• Page Start: 705
• Page End: 713
• Publication Date: 2019-04
• Subjects: Phosphatidylinositol 3-kinase regulatory subunit gamma -- Nile tilapia -- Expression pattern -- Immune response -- Streptococcus agalactiae; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.01.006
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: A full-length cDNA encoding phosphatidylinositol 3-kinase regulatory subunit gamma b gene in Nile tilapia ( Oreochromis niloticus ), termed as On-pik3r3b, was identified and characterized in this study. The sequence analysis demonstrated that the full-length cDNA of On-pik3r3b was 2018 bp, containing a 5′ untranslated region (UTR) of 171 bp, an open reading frame (ORF) of 1422 bp and a 3' UTR of 425 bp. Its protein sequence displayed a high degree of identity with other fish. Using qPCR, the expression patterns of On-pik3r3b were investigated. In healthy Nile tilapia, the transcripts of On-pik3r3b were detected in all examined tissues, except the skin. Upon the stimulation with Streptococcus agalactiae, the On-pik3r3b expression level in liver, spleen, kidney and gill were significantly increased at 12 h after infection. The recombinant On-pik3r3b showed in vitro antibacterial activity, against S. agalactiae and E. coli . Our observation strongly indicates that On-pik3r3b is involved in the innate immune response in Nile tilapia.