Nucleation and Growth of Amino Acid and Peptide Supramolecular Polymers through Liquid–Liquid Phase Separation. Issue 50 (7th November 2019)

• Record Type: Journal Article
• Authors: Yuan, Chengqian; Levin, Aviad; Chen, Wei; Xing, Ruirui; Zou, Qianli; Herling, Therese W.; Challa, Pavan Kumar; Knowles, Tuomas P. J.; Yan, Xuehai
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 50(2019)
• Page Start: 18116
• Page End: 18123
• Publication Date: 2019-11-07
• Subjects: amino acids -- liquid–liquid phase separation -- nucleation -- peptides -- supramolecular polymers; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201911782
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The transition of peptides and proteins from the solution phase into fibrillar structures is a general phenomenon encountered in functional and aberrant biology and is increasingly exploited in soft materials science. However, the fundamental molecular events underpinning the early stages of their assembly and subsequent growth have remained challenging to elucidate. Here, we show that liquid–liquid phase separation into solute‐rich and solute‐poor phases is a fundamental step leading to the nucleation of supramolecular nanofibrils from molecular building blocks, including peptides and even amphiphilic amino acids. The solute‐rich liquid droplets act as nucleation sites, allowing the formation of thermodynamically favorable nanofibrils following Ostwald's step rule. The transition from solution to liquid droplets is entropy driven while the transition from liquid droplets to nanofibrils is mediated by enthalpic interactions and characterized by structural reorganization. These findings shed light on how the nucleation barrier toward the formation of solid phases can be lowered through a kinetic mechanism which proceeds through a metastable liquid phase. Abstract : Separation into rich and poor : The formation of supramolecular nanofibrils from amphiphilic amino acids and short peptides follows a nucleation–elongation mechanism mediated by liquid–liquid phase separation. The initial formation of solute‐poor and solute‐rich liquid droplets is entropy driven, while the transition of liquid droplets to nanofibrils is dominated by enthalpic interactions.