Biological synthesis of silver nanoparticles by cell‐free extract of Polysiphonia algae and their anticancer activity against breast cancer MCF‐7 cell lines. (1st May 2019)

• Record Type: Journal Article
• Authors: Moshfegh, Azam; Jalali, Amir; Salehzadeh, Ali; Sadeghi Jozani, Amin
• Is Part Of: Micro & nano letters. Volume 14:Number 5(2019)
• Page Start: 581
• Page End: 584
• Publication Date: 2019-05-01
• Subjects: nanoparticles -- cancer -- scanning electron microscopy -- nanofabrication -- transmission electron microscopy -- surface plasmon resonance -- ultraviolet spectra -- biomedical materials -- nanomedicine -- X‐ray chemical analysis -- cellular biophysics -- toxicology -- visible spectra -- antibacterial activity -- particle size -- silver -- Fourier transform infrared spectra -- microorganisms; biological synthesis -- silver nanoparticles -- polysiphonia algae -- anticancer activity -- breast cancer MCF‐7 cell line -- green nanomaterial production techniques -- medicinal applications -- biocompatibility -- toxic by‐products -- silver nitrate solutions -- yellow‐brown colour formation -- surface plasmon resonance -- green synthesised AgNPs -- scanning electron microscopy -- transmission electron microscopy images -- spherical AgNPs -- synthesised nanoparticles -- anticancer AgNPs -- cancerous cells -- cell‐free extract -- ultraviolet‐visible spectroscopy -- energy‐dispersive X‐ray analysis -- reducing agent -- Fourier‐transform infrared spectroscopy -- FTIR spectroscopy -- MTT assay -- flow cytometry -- inhibitory activity -- filamentous red alga Polysiphonia extract -- size 5.0 nm to 25.0 nm -- wavelength 420.0 nm -- Ag; Nanotechnology -- Periodicals; Nanostructures -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1049/mnl.2018.5260
• Languages: English
• ISSNs: 1750-0443

Abstract

Abstract : Green nanomaterial production techniques are desired for medicinal applications because of their biocompatibility and lack of toxic by‐products. This work reports the biological synthesis of silver nanoparticles (AgNPs) from silver nitrate solutions using the cell‐free extract of Polysiphonia alga as a reducing agent. The visible yellow‐brown colour formation and surface plasmon resonance at 420 nm by ultraviolet–visible spectroscopy indicates the reduction of silver nitrate and biosynthesis of AgNPs. For more characterisation of green synthesised AgNPs, Fourier‐transform infrared (FTIR) spectroscopy analysis is used and the results confirmed the presence of functional groups capping in the AgNPs. The scanning electron microscopy and transmission electron microscopy images showed mostly spherical AgNPs with a size range between 5 and 25 nm and the concentration of 3.1 × 10 6 particles/ml. The results of energy‐dispersive X‐ray analysis confirmedthe presence of Ag in the synthesised nanoparticles. The MTT assay and flow cytometry are used to determine the toxicity of AgNPs against breast cancer MCF‐7 cell line and the results showed the best inhibitory activity at 100 µg/ml. Generally, green synthesis of anticancer AgNPs using filamentous red alga Polysiphonia extract can easily be scaled up for many biomedical applications such as defence against the cancerous cells.