Antimicrobial and anthelmintic activities of the ethanolic extract, fractions and isolated compounds from Manilkara zapota L. P. Royen (Sapotaceae). (4th February 2021)

• Record Type: Journal Article
• Authors: Mourão Mulvaney, Layany Carolyny; Xavier-Júnior, Francisco Humberto; Rodrigues, Alice M S; Stien, Didier; Allegretti, Silmara Marques; Garcia, Vera Lúcia
• Is Part Of: Journal of pharmacy and pharmacology. Volume 73:Number 3(2021)
• Page Start: 377
• Page End: 387
• Publication Date: 2021-02-04
• Subjects: traditional medicine meso- and Southern America -- phytochemistry -- anthelmintic -- antimicrobial -- chromatography; Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1093/jpp/rgaa030
• Languages: English
• ISSNs: 0022-3573

Abstract

Abstract: Objectives: To evaluate the antibacterial, antifungal and anthelmintic activities of the ethanolic extract (EEMz), fractions (LPFMz and HPFMz) and compounds isolated from the leaves of Manilkara zapota L. P. Royen. Methods: Extract and fractions were produced by turbolization. LPFMz fraction was analysed by gas chromatography–mass spectrometry. The isolated compounds from HPFMz were purified by flash and preparative chromatographic methods, and chemically characterised by UPLC-ESITOFMS, optical rotation, and one- and two-dimensional 1 H and 13 C NMR techniques. Anthelmintic against Strongyloides venezuelensis and antimicrobial activities against Candida albicans, Trichophyton rubrum and Staphylococcus aureus were evaluated. Key findings: EEMz showed mainly phenolic compounds and pentacyclic triterpenes from Δ12-oleane/Δ12-ursane series. Chlorogenic acid, myricetin-3-O-β-D-glucopyranoside, mearnsitrin, germanicol and germanicol acetate were reported to M. zapota leaves for the first time in this work. EEMz, HPFMz, LPFMz showed significative activity against C. albicans (16 µg/mL), while isolated flavonoids were active against S. aureus (<32 µg/mL). EEMz, phenolic-rich compounds (F2 ), and chlorogenic acid were potentially active against S. venezuelensis at 24 h. Conclusions: M. zapota and its bioactive compound can be eligible such as a potential phytomedicine for the treatment of microbial and strongyloidiasis drug-resistant infections.