Antifungal and cytotoxicity activities of Banisteriopsis argyrophylla leaves. (23rd August 2018)

• Record Type: Journal Article
• Authors: Oliveira, Daiane M; Silva, Tomás F R; Martins, Mário M; de Morais, Sérgio A L; Chang, Roberto; de Aquino, Francisco J T; da Silva, Claudio V; Teixeira, Thaise L; Martins, Carlos H G; Moraes, Thaís S; Cunha, Luís C S; Pivatto, Marcos; de Oliveira, Alberto
• Is Part Of: Journal of pharmacy and pharmacology. Volume 70:Number 11(2018)
• Page Start: 1541
• Page End: 1552
• Publication Date: 2018-08-23
• Subjects: antifungal -- Banisteriopsis argyrophylla -- Cerrado -- cytotoxicity -- flavonoids; Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12996
• Languages: English
• ISSNs: 0022-3573

Abstract

Abstract: Objectives: This work aimed to evaluate the antifungal and cytotoxic activity of the EtOH extract and fractions of Banisteriopsis argyrophylla leaves, and to perform the identification of these bioactive metabolites. Methods: The EtOAc fraction (EAF) obtained from the ethanolic extract of B. argyrophylla leaves showed better antifungal potential against Candida spp. In this fraction, ten flavonoids have been identified by UHPLC-ESI-MS n . Then, EAF was submitted to column chromatography to give four new fractions (A1–A4). The cytotoxicity was determined against Vero cells. Key findings: The EAF showed better antifungal potential against Candida spp. with minimum inhibitory concentrations (MICs) between 31.25 and 93.75 μg/ml. The (–)-catechin (fraction A1) showed a MIC of 2.83 μg/ml against Candida glabrata . Fractions A2, A3 and A4 were rich in quercetins and kaempferols and showed good inhibitory concentrations (5.86–46.87 μg/ml) against C. albicans, C. glabrata and C. tropicalis . Conclusions: The EtOH extract, fractions and the isolated (–)-catechin showed lower toxicity to Vero cells than cisplatin, used as a positive control. Thus, the leaves of B. argyrophylla are a promising source of antifungal agents.