Antihyperglycaemic activity of the methanol extract from leaves of Eremophila maculata (Scrophulariaceae) in streptozotocin-induced diabetic rats. (21st March 2017)

• Record Type: Journal Article
• Authors: Youssef, Fadia S; Ashour, Mohamed L; Ebada, Sherif S; Sobeh, Mansour; El-Beshbishy, Hesham A; Singab, Abdel Nasser; Wink, Michael
• Is Part Of: Journal of pharmacy and pharmacology. Volume 69:Number 6(2017:Jun.)
• Page Start: 733
• Page End: 742
• Publication Date: 2017-03-21
• Subjects: antihyperglycaemic -- Eremophila maculata -- molecular modelling -- Scrophulariaceae -- streptozotocin; Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12690
• Languages: English
• ISSNs: 0022-3573

Abstract

Abstract: Objectives: This study was designed to evaluate the antihyperglycaemic activity of the methanol leaf extract of Eremophila maculata (EMM) both in vitro and in vivo . Methods: The antihyperglycaemic activity was assessed in vitro using differentiated 3T3-L1 adipocytes, whereas in-vivo effect was evaluated in streptozotocin-induced diabetic rats. Chemical profiling of EMM was done using LC-ESI-MS techniques. Molecular modelling experiments of the identified compounds were performed using C-Docker protocol. Key findings: Eremophila maculata slightly enhanced cellular glucose uptake and utilization in vitro by 3.92% relative to the untreated control. A stronger in-vivo effect was observed for EMM and its dichloromethane fraction. A pronounced elevation in serum insulin by 88.89 and 66.67%, respectively, accompanied by an apparent decline in fasting blood glucose (FBG) level by 65.60 and 70.37% comparable to streptozotocin-induced diabetic rats was observed. This effect was stronger than that of the reference drug glibenclamide (GLB). Chemical profiling of EMM revealed that leucoseptoside A, verbascoside, syringaresinol-4- O- β-D-glucopyranoside, pinoresinol-4 -O- β-D-glucopyranoside and pinoresinol-4- O -[6″- O -( E )-feruloyl]-β-D-glucopyranoside are the major compounds. Molecular modelling showed that martynoside, verbascoside and phillygenin exhibited the highest inhibition to human pancreatic α-amylase (HPA), maltase glucoamylase (MGAM) and aldose reductase (AR), respectively. Conclusion: Eremophila maculata offers an interesting relatively safer antihyperglycaemic candidate comparable to synthetic analogues.