Effects of dendrimer/cyclodextrin conjugates as gene transfer carriers on nitric oxide production from macrophages. (16th June 2015)

• Record Type: Journal Article
• Authors: Motoyama, Keiichi; Sako, Asumi; Ibrahim Abu Hashim, Irhan; Higashi, Taishi; Arima, Hidetoshi
• Is Part Of: Journal of pharmacy and pharmacology. Volume 68:Number 5(2016:May)
• Page Start: 598
• Page End: 607
• Publication Date: 2015-06-16
• Subjects: cyclodextrin -- dendrimer -- gene transfer carrier -- nitric oxide -- toll-like receptor ligands; Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12439
• Languages: English
• ISSNs: 0022-3573

Abstract

Abstract: Objectives: The development of safe gene transfer carriers with high transfection efficiency, which does not affect the cell function, is a challenging issue. In this study, we examined the effects of α-cyclodextrin (α-CyD)/dendrimer conjugate (α-CDE (G3)) on nitric oxide (NO) production in murine macrophages J774.1 cells stimulated with toll-like receptors (TLR) ligands. Methods: NO production from macrophages stimulated with TLR ligands was determined by the Griess method. Transfection efficiency of α-CDE (G3)/plasmid DNA (pDNA) complex was quantified by a luminometer. Key findings: α-CDE (G3) significantly inhibited NO production from J774.1 cells stimulated with TLR ligands. α-CyD molecules in α-CDE (G3) had no effect on NO production. The inhibitory effect of α-CDE (G3) on NO production might be attributed to the dendrimer (G3). Increasing the degree of substitution (DS) of α-CyD in the α-CDE (G3) molecule was accompanied by a significant decrease in the inhibition of NO production. Furthermore, higher gene transfection efficiency of α-CDE (G3)/pDNA complex was observed upon increasing the DS of α-CyD. Conclusions: α-CDE (G3) with high DS value of α-CyD may be considered as a safe gene transfer carrier that does not adversely affect NO production from macrophages stimulated with TLR ligands.