Identification of viral particles in the apoplast of Nicotianabenthamiana leaves infected by potato virus X. (24th February 2021)

• Record Type: Journal Article
• Authors: Hu, Shuzhen; Yin, Yueyan; Chen, Buyang; Lin, Qi; Tian, Yanzhen; Song, Xijiao; Peng, Jiejun; Zheng, Hongying; Rao, Shaofei; Wu, Guanwei; Mo, Xiaohan; Yan, Fei; Chen, Jianping; Lu, Yuwen
• Is Part Of: Molecular plant pathology. Volume 22:Number 4(2021)
• Page Start: 456
• Page End: 464
• Publication Date: 2021-02-24
• Subjects: apoplast -- coat protein -- exosomes -- potato virus X -- viral particles; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.13039
• Languages: English
• ISSNs: 1464-6722

Abstract

Abstract: The apoplast is the extracellular space for signalling, nutrient transport, and plant–microbe interactions, but little is known about how plant viruses use the foliar apoplast. Proteomic analysis of the apoplasts isolated from potato virus X (PVX)‐infected Nicotiana benthamiana plants showed that the coat protein (CP) is the dominant viral component. The presence of the CP in the apoplast was confirmed by western blot, viral nucleic acid was detected by reverse transcription‐PCR and northern blot, and viral particles were observed by transmission electron microscopy (TEM). The apoplast from infected leaves was infectious if rubbed onto healthy leaves but not when infiltrated into them. The exosomes were separated from the apoplast fluid by high‐speed centrifugation and TEM showed that PVX particles were not associated with the exosomes. These results suggest that PVX virions are released to the N. benthamiana apoplast in a one‐way manner and do not share the bidirectional transport of exosomes. Abstract : The presence of potato virus X (PVX) viral particles was identified in the leaf apoplast isolated from PVX‐infected Nicotiana benthamiana . This apoplast was infectious onto healthy leaves by rubbing them but not by infiltration.