Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017. (1st April 2021)

• Record Type: Journal Article
• Authors: Dieng, Idrissa; Diarra, Maryam; Diagne, Moussa Moïse; Faye, Martin; Dior Ndione, Marie Henriette; Ba, Yamar; Diop, Mamadou; Ndiaye, El Hadji; Marinho de Andrade Zanotto, Paolo; Diop, Boly; Ndiaye, Mamadou; Bousso, Abdoulaye; Dia, Ndongo; Diallo, Mawlouth; Barry, Aliou; Fall, Gamou; Loucoubar, Cheikh; Sall, Amadou Alpha; Faye, Ousmane; Faye, Oumar
• Other Names: J. Gonzalez Jean-Paul Academic Editor.
• Is Part Of: Journal of tropical medicine. Volume 2021(2021)
• Publication Date: 2021-04-01
• Subjects: Tropical medicine -- Periodicals; 616.9883
• DOI: 10.1155/2021/8817987
• Languages: English
• ISSNs: 1687-9686

Abstract

Abstract : Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management.