Debugging: putting the synthetic yeast chromosome to work. Issue 15 (15th March 2021)

• Record Type: Journal Article
• Authors: Xie, Ze-Xiong; Zhou, Jianting; Fu, Juan; Yuan, Ying-Jin
• Is Part Of: Chemical science. Volume 12:Issue 15(2021)
• Page Start: 5381
• Page End: 5389
• Publication Date: 2021-03-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0sc06924h
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : This review summarizes strategies used to map and repair various bugs in synthetic genomic sequences and provides guidance for the construction of synthetic yeast chromosomes that are capable of maintaining cell fitness. Abstract : Synthetic genomics aims to de novo synthesize a functional genome redesigned from natural sequences with custom features. Designed genomes provide new toolkits for better understanding organisms, evolution and the construction of cellular factories. Currently maintaining the fitness of cells with synthetic genomes is particularly challenging as defective designs and unanticipated assembly errors frequently occur. Mapping and correcting bugs that arise during the synthetic process are imperative for the successful construction of a synthetic genome that can sustain a desired cellular function. Here, we review recently developed methods used to map and fix various bugs which arise during yeast genome synthesis with the hope of providing guidance for putting the synthetic yeast chromosome to work.