CircNFIX promotes progression of pituitary adenoma via CCNB1 by sponging miR-34a -5p. (5th April 2021)

• Record Type: Journal Article
• Authors: Cheng, Jianhua; Nie, Ding; Li, Bin; Gui, SongBai; Li, ChuZhong; Zhang, YaZhuo; Zhao, Peng
• Is Part Of: Molecular and cellular endocrinology. Volume 525(2021)
• Publication Date: 2021-04-05
• Subjects: CircNFIX -- MiR-34a-5p -- CCNB1 -- Progression -- Pituitary adenoma; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2020.111140
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Previous studies have shown that CCNB1 affects the invasiveness of pituitary adenomas, and it is of great significance to find the upstream mechanism of regulating CCNB1.In this study, we explored a significantly overexpressed circRNA in invasive pituitary adenomas. Based on bioinformatics analysis and mechanism experiments, we determined that circNFIX (has-circ_0005660) affects cell invasion, migration and proliferation in pituitary adenomas by sponging miR-34a-5p through CCNB1. In pituitary adenoma tissues, the expression of circNFIX and CCNB1 was upregulated, while miR-34a-5p expression was downregulated. The silencing of circNFIX or overexpression of miR-34a-5p inhibited cell invasion, migration and proliferation. Inhibition of miR-34a-5p expression reversed the inhibitory effect of circNFIX silencing on the progression of pituitary adenoma. In conclusion, CircNFIX affects cell invasion, migration, and proliferation in pituitary adenomas by sponging miR-34a-5p through CCNB1. Therefore, circNFIX is expected to serve as a potential target for the treatment of pituitary adenomas.