Advances in single-cell multi-omics profiling. Issue 2 (20th January 2021)

• Record Type: Journal Article
• Authors: Bai, Dongsheng; Peng, Jinying; Yi, Chengqi
• Is Part Of: RSC chemical biology. Volume 2:Issue 2(2021)
• Page Start: 441
• Page End: 449
• Publication Date: 2021-01-20
• Subjects: 572
• DOI: 10.1039/d0cb00163e
• Languages: English
• ISSNs: 2633-0679

Abstract

Abstract : Single-cell multi-omics profiling methods are developed to dissect heterogeneity of cell populations. Abstract : Single-cell profiling methods are developed to dissect heterogeneity of cell populations. Recently, multiple enzymatic or chemical treatments have been integrated into single-cell multi-omics profiling methods with high compatibility. These methods have been verified to identify rare or new cell types with high confidence. Single-cell multi-omics analysis can also provide tools to solve the complex regulatory network associated with genome coding, epigenome regulation, and transcripotome expression in a single cell. However, acquiring high-quality single-cell data still faces inherent technical challenges, and co-assays with some other layers of cell identify such as transcription factors binding, histone modifications etc ., profiles need new technological breakthroughs to facilitate a more thorough understanding of a single cell. In this review, we summarize the recent advances of single-cell multi-omics methods and discuss the challenges and opportunities in this filed.