Functionalized silica-based nanoparticles for photodynamic therapy. (August 2011)

• Record Type: Journal Article
• Authors: Couleaud, Pierre; Bechet, Denise; Vanderesse, Régis; Barberi-Heyob, Muriel; Faure, Anne-Charlotte; Roux, Stéphane; Tillement, Olivier; Porhel, Sabine; Guillemin, François; Frochot, Céline
• Is Part Of: Nanomedicine. Volume 6:Number 6(2011)
• Page Start: 995
• Page End: 1009
• Publication Date: 2011-08
• Subjects: functionalized-nanoparticle -- in vitro -- molecular affinity -- neuropilin-1 -- peptide -- photodynamic efficiency; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.11.31
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: The strategy developed aims to favor the vascular effect of photodynamic therapy by targeting tumor-associated vascularization using peptide-functionalized nanoparticles. We previously described the conjugation of a photosensitizer to a peptide targeting neuropilin-1 overexpressed in tumor angiogenic vessels.Materials & Methods: In this study, we have designed and photophysically characterized a multifunctional nanoparticle consisting of a surface-localized tumor vasculature targeting peptides and encapsulated photodynamic therapy and imaging agents.Results & Conclusion: The elaboration of these multifunctional silica-based nanoparticles is reported. Nanoparticles functionalized with approximately 4.2 peptides bound to recombinant neuropilin-1 protein. Nanoparticles conferred photosensitivity to cells overexpressing neuropilin-1, providing evidence that the chlorin grafted within the nanoparticle matrix can be photoactivated to yield photocytotoxic effects in vitro .