Novel synthesis of cerium oxide nanoparticles for free radical scavenging. (June 2007)

• Record Type: Journal Article
• Authors: Tsai, Yi-Yang; Oca-Cossio, Jose; Agering, Kristina; Simpson, Nicholas E; Atkinson, Mark A; Wasserfall, Clive H; Constantinidis, Ioannis; Sigmund, Wolfgang
• Is Part Of: Nanomedicine. Volume 2:Number 3(2007)
• Page Start: 325
• Page End: 332
• Publication Date: 2007-06
• Subjects: antioxidant -- cerium oxide -- free radical scavenging -- nanoparticles -- reverse micelle method; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/17435889.2.3.325
• Languages: English
• ISSNs: 1743-5889

Abstract

Aims: The aim of this article is to present a novel synthetic route to form CeO2 nanoparticles that protects against the detrimental influence of oxidative stress in mammalian cells.Methods: The noncytotoxic surfactant lecithin was used to synthesize CeO2 nanoparticles and the products were colloidally stabilized in a biocompatible tri-sodium citrate buffer. These nanoparticles were delivered into murine insulinoma ββTC-tet cells, and intracellular free radical concentrations responding to exposure to hydroquinone were measured in a variety of extracellular CeO2 concentrations.Results: Well-dispersed, highly crystallized CeO2 nanoparticles of 3.7 nm in size were achieved that are chemically and colloidally stable in Dulbecco''s modified Eagle''s medium for extended periods of time. Treating ββTC-tet cells with these nanoparticles alleviated detrimental intracellular free radical levels down to the primary level.Conclusion: CeO2 nanoparticles synthesized from this route are demonstrated to be effective free radical scavengers within ββTC-tet cells. Furthermore, it is shown that CeO2 nanoparticles provide an effective means to improve cellular survival in settings wherein cell loss due to oxidative stress limits native function.