Developmental origins of ovarian disorder: impact of maternal lean gestational diabetes on the offspring ovarian proteome in mice. Issue 4 (10th July 2019)

• Record Type: Journal Article
• Authors: Clark, Kendra L; Talton, Omonseigho O; Ganesan, Shanthi; Schulz, Laura C; Keating, Aileen F
• Is Part Of: Biology of reproduction. Volume 101:Issue 4(2019)
• Page Start: 771
• Page End: 781
• Publication Date: 2019-07-10
• Subjects: gestational diabetes -- ovary -- proteomics -- offspring health; Reproduction -- Periodicals; Biology; Reproduction; Reproduction; Electronic journals; Periodicals; Periodicals; 571.805
• DOI: 10.1093/biolre/ioz116
• Languages: English
• ISSNs: 0006-3363

Abstract

Abstract: Gestational diabetes mellitus (GDM) is an obstetric disorder affecting approximately 10% of pregnancies. The four high-fat, high-sucrose (HFHS) mouse model emulates GDM in lean women. Dams are fed a HFHS diet 1 week prior to mating and throughout gestation resulting in inadequate insulin response to glucose in mid-late pregnancy. The offspring of HFHS dams have increased adiposity, thus, we hypothesized that maternal metabolic alterations during lean GDM would compromise ovarian function in offspring both basally and in response to a control or HFHS diet in adulthood. Briefly, DL PL were lean dams and control diet pups; DL PH were lean dams and HFHS pups; DH PL were HFHS dams and control diet pups; and DH PH were HFHS dams and HFHS pups. A HFHS challenge in the absence of maternal GDM (DL PL vs. DL PH ) increased 3 and decreased 30 ovarian proteins. Maternal GDM in the absence of a dietary stress (DL PL vs. DH PL ) increased abundance of 4 proteins and decreased abundance of 85 proteins in the offspring ovary. Finally, 87 proteins increased, and 4 proteins decreased in offspring ovaries due to dietary challenge and exposure to maternal GDM in utero (DL PL vs. DH PH ). Canopy FGF signaling regulator 2, deleted in azoospermia-associated protein 1, septin 7, and serine/arginine-rich splicing factor 2 were altered across multiple offspring groups. Together, these findings suggest a possible impact on fertility and oocyte quality in relation to GDM exposure in utero as well as in response to a western diet in later life. Abstract : Summary Sentence Altered abundance of ovarian proteins in offspring who experienced maternal GDM highlights the potential long-term effects of metabolic changes on ovarian function.