Preparation, characterisation and antibacterial activity of a florfenicol‐loaded solid lipid nanoparticle suspension. Issue 6 (1st December 2015)

• Record Type: Journal Article
• Authors: Wang, Ting; Chen, Xiaojin; Lu, Mengmeng; Li, Xihe; Zhou, WenZhong
• Is Part Of: IET nanobiotechnology. Volume 9:Issue 6(2015)
• Page Start: 355
• Page End: 361
• Publication Date: 2015-12-01
• Subjects: antibacterial activity -- suspensions -- nanomedicine -- nanoparticles -- heat treatment -- veterinary medicine -- toxicology; suspension preparation -- suspension characterisation -- antibacterial activity -- florfenicol‐loaded solid lipid nanoparticle suspension -- FFC‐SLN -- hot homogenisation -- ultrasonic technique -- release profile -- stability -- physicochemical properties -- nanoparticles -- polydispersity index -- zeta potential -- minimum inhibition concentration values -- Staphylococcus aureus -- Escherichia coli -- native florfenicol -- cytotoxicity -- mortality protection -- lethal infection -- veterinary medicine; Biotechnology -- Periodicals; Nanotechnology -- Periodicals; 660.6
• DOI: 10.1049/iet-nbt.2015.0012
• Languages: English
• ISSNs: 1751-8741

Abstract

Abstract : A florfenicol‐loaded solid lipid nanoparticle (FFC‐SLN) suspension was prepared by hot homogenisation and ultrasonic technique. The suspension was characterised for its release profile, stability, toxicity, and the physicochemical properties of the nanoparticles. Antibacterial activity of the suspension was evaluated in vitro and in vivo . The results showed that the mean diameter, polydispersity index and zeta potential of the nanoparticles were 253 ± 3 nm, 0.409 ± 0.022 and 47.5 ± 0.21 mV, respectively. In vitro release profile showed the FFC‐SLN suspension had sustained release effect. The minimum inhibition concentration values of the FFC‐SLN suspension were 6 and 3 µg/mL against Staphylococcus aureus and Escherichia coli respectively, compared with 3.5 and 2 µg/mL of native florfenicol. The suspension was relatively stable at 4°C and less stable at room temperature during 9 months storage. Although the nanoparticle carriers exhibited cytotoxicity in cell cultures, the LD50 of the lyophilised dry power of the suspension was higher than 5 g/kg body weight. Mortality protection against E. coli lethal infection in mice showed that the nanoparticle suspension had much better efficacy (6/10) than native drug (1/10). These results indicate that FFC‐SLN suspension could be a promising formulation in veterinary medicine.